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Kusnoto ¹, Setiawan Koesdarto ¹, Sri Mumpuni ¹, R. Heru Prasetyo ¹

Affiliations
1 Department of Parasitology, Universitas Airlangga, Surabaya, East Java, ID

Abstract

Aim: The aim of this study was to detect specific immunoglobulin (Ig) that could be used to determine monoclonal antibody in conjugate-making an effort for the indirect enzyme-linked immunosorbent assay (ELISA) diagnostic kit of toxocariasis in human.
Materials and Methods: The study was conducted to assess the Ig profile, based on ELISA-isotyping, in mice infected with second stage larvae eggs of Toxocara cati. The optical density values of anti-T. cati mice serum IgG subclasses were analyzed by applying ANOVA factorial.
Results: The specific IgG subclass in mice infected with T. cati mice was found to be IgG2β.
Conclusion: Subclass of IgG, especially IgG2β, can provide leads about the use of the monoclonal antibody in conjugate making an effort for the indirect ELISA diagnostic kit.

Keywords

Diagnostic Kit, Enzyme-Linked Immunosorbent Assay, Human, Immunoglobulin G Subclass, Monoclonal Antibody, Toxocariasis, Toxocara cati.

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Muhammad Rama Imam Saputra ¹, April H. Wardhana ², Mufasirin ³, Setiawan Koesdarto ³, Lucia Tri Suwanti ³, Nunuk Dyah Retno Lastuti ³, Endang Suprihati ³

Affiliations
1 Disease and Veteriner Public Health Science, Airlangga University, ID
2 Department of Parasitology, Veterinery Center of Bogor, ID
3 Department of Parasitology, Airlangga University, ID

Abstract

Trypanosomiasis or surra is a disease caused by blood parasite of Trypanosoma evansi. Trypanosomiasis disease in Indonesia includes Strategic Diseases of Infectious Diseases (SDID). The purpose of this study is to detect Trypanosomiasis in horses in Bima, Indonesia. A total of 90 horse blood samples were used for the study. Sample examination using blood smears technique with Giemsa staining and Card Agglutination Test of Trypanosoma evansi (CATT). The result of examination of blood samples by microscope obtained 3 positive samples infected T. evansi and by CATT obtained 1 positive samples containing antibodies against T. evansi. It was concluded that trypanosomiasis in horses in Bima was 3.3%.

Keywords

Bima, Blood Smears, Card Agglutination Test Of Trypanosoma Evansi (CATT), Horse, Trypanosoma Evansi.

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